P612
886-2-2789-6761
cfchou [at] phys.sinica.edu.tw
P612
886-2-2789-6761
cfchou [at] phys.sinica.edu.tw
Chu, Michelle / 886-2-2789-8364
(1) | 國內學術研究獎項 | 2014-04 | 2013 科技部傑出研究獎 Outstanding Research Award of Ministry of Science and Technology |
(1) | 西元年:2012 研究人員(中):周家復、葉佳唯、Alessandro Taloni、陳彥龍 研究人員(英):CHOU, CHIA-FU, Jia-Wei Yeh, Alessandro Taloni, Yeng-Long Chen 研究成果名稱(中):微奈米流道介面熵力驅動之單分子DNA拔河 研究成果名稱(英):Entropy-driven single molecule tug-of-war of DNA at micro-nanofluidic interfaces 簡要記述(中):熵驅動的高分子動力學在生物系統中是極其重要的,但在奈米尺度上的熵力及其對奈米侷限度的相依性仍然是不清楚的。在這裡,我們建立了一個由熵力驅動的單分子DNA拔河系統,該系統由一個奈米狹縫橋接兩個微奈米流道的界面組成。由此系統,我們在不需施加外力的情況下,便可研究奈米尺度下,生物分子的熵力及其對奈米侷限度的相依性及其規度律。我們的結果提供了直接的實驗證據表明,熵力祇和分子在奈米尺度的空間侷限(即狹縫高度)相關,而和狹縫長度和在其內的DNA長度無關。我們的研究結果對高分子聚合物在奈米尺度的傳輸現象,單分子分析的系統設計上,及生物科技上均有潛在的應用性。 簡要記述(英):Entropy-driven polymer dynamics at the nanoscale is fundamentally important in biological systems but the dependence of the entropic force on the nanoconfinement remains elusive. Here we established an entropy-driven single molecule tug-of-war (TOW) at two micro-nanofluidic interfaces bridged by a nanoslit, performed the force analysis from a modified worm-like chain in the TOW scenario and the entropic recoiling process, and determined the associated scalings on the nanoconfinement. Our results provide a direct experimental evidence that the entropic forces in these two regimes, though unequal, are essentially constant at defined slit heights, irrespective of the slit lengths and the DNA segments within. Our findings have the implications to polymer transport at the nanoscale, device design for single molecule analysis, and biotechnological applications.
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(2) | 西元年:2012 研究人員(中):周家復、廖國棠 研究人員(英):CHOU, CHIA-FU, Kuo-Tang Liao 研究成果名稱(中):奈米分子阱和分子壩應用於高導電度緩衝液內超快速之蛋白質富集(濃縮) 研究成果名稱(英):Nanoscale molecular traps and dams for ultrafast protein enrichment in high-conductivity buffers 簡要記述(中):我們提出一個新的分子富集的方法,稱為分子壩,該方法可在生理緩衝液內,以奈米尺度的無電極式介電泳,快速達到蛋白質富集與濃縮。我們以絕緣材料製作了奈米流道,並在其中嵌製30奈米大小的間隙,作為聚焦電場的透鏡,在結合微奈米流道界面時,可將外加電場放大約10萬倍。藉由這個奈米間隙聚焦的強電場和其場梯度,並利用分子壩的效應,我們達到快速濃縮蛋白質的效果,在20秒內便可濃縮至少10萬倍,比以往文獻報告的方法快了幾個數量級。我們的研究開闢了奈米級分子水壩的可能應用,包括以微型化感測平台快速且靈敏的分析蛋白質和發現生物標誌物,或應用於沉澱研究和蛋白質結晶學,並可能進一步擴展到小分子的富集或篩選。 簡要記述(英):We report a new approach, termed molecular dam, to enhance mass transport for protein enrichment in nanofluidic channels by nanoscale electrodeless dielectrophoresis under physiological buffer conditions. Dielectric nanoconstrictions, down to 30nm in size, embedded in nanofluidic device, serve as field focusing lens capable of magnifying the applied field to 100000-fold when combined with a micro-to-nanofluidic step interface. Empowered by this strong field and the associated field gradient occurred at the nanoconstrictions, we demonstrate proteins are enriched by molecular damming effect, faster than the trapping effect, to greater than 100000-fold in 20 seconds, which is orders of magnitude faster than most reported methods. Our study opens up further possibilities of using nanoscale molecular dams in miniaturized sensing plat-forms for rapid and sensitive protein analysis and biomarker discovery, with potential applications in precipitation studies and protein crystallization, and possible extensions to small molecules enrichment or screening.
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