302-1
886-2-2789-6763
khlin [at] phys.sinica.edu.tw
302-1
886-2-2789-6763
khlin [at] phys.sinica.edu.tw
Rao, Cindy / 886-2-2789-8916
(1) | 國內學術研究獎項 | 2016-12 | 國家新創獎-最佳產業效益獎 | |
(2) | 國內學術研究獎項 | 2013-03 | 台灣女科學家新秀獎 (Taiwan Outstanding Young Female Scientist Award) | |
(3) | 其他國際學術研究獎項 | 2011-08 | TWAS Young Affiliates |
(1) | 西元年:2013 研究人員(中):林耿慧、李怡萱 研究人員(英):LIN, KENG-HUI, Yi-hsuan Lee 研究成果名稱(中):藉由可調節軟硬度的三維均一孔洞鷹架來了解細胞在三維感測的軟硬度 研究成果名稱(英):The response of cell to the stiffness of 3D compliant scaffold of uniform pores 簡要記述(中):設計一個適合在三維環境研究細胞與基材的鷹架是了解三維細胞行為的關鍵. 過去十幾年因為新型可調節軟硬度的二維細胞培養基材的發明, 帶動科學家對細胞力學的研究, 我們把這可調節軟硬度的基材做成三維.我們利用纖維母細胞作為模型系統, 藉由分析他們型態的伸長量, 來量化他們對周圍環境軟硬度的反應. 我們也發現在洞裡, 細胞黏著分子與骨架的分布與二維的細胞很不同. 簡要記述(英):Designing biomaterials for studying cell-extracellular matrix (ECM) interactions in three dimensions (3D) is key to the biological relevance of observations of cells grown in 3D culture. In recent decades, novel two-dimensional substrates such as compliant gels with patterned proteins have provided many useful insights into how adhesive and mechanical cues drive cellular behavior. Here, we extend cell culture into the third dimension by engineering uniform pores in compliant gels; these pores are treated with fibronectin to pattern ECM proteins as a spherical shell. The rigidity of the 3D microenvironment is controlled by choice of base gels used to assemble the scaffolds. Fibroblasts exhibit quantitative differences in morphology and cytoskeletal architecture following culture in our 3D scaffolds versus 2D substrates. Our new technology offers independent control over factors such as three-dimensionality, curvature, biochemical composition, and the mechanical stiffness of the substrate, all of which make critical contributions to the formation of cell adhesions in 3D.
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(2) | 西元年:2010 研究人員(中):林耿慧 研究人員(英):LIN, KENG-HUI 研究成果名稱(中):不同組織細胞在相同三維圓形孔洞材料的型態與組織 研究成果名稱(英):Organization and Morphology of Tissue Cells in Ordered Cellular Solids 簡要記述(中):我們改進之前微流體通道製作三維鷹架的製作 - 選擇細胞喜歡貼覆的吉利丁材質, 並且改變微流體通道的製作與收集方法. 這樣製作出來的鷹架能有更大的範圍. 我們在相同的三維微環境中養三種不同組織的細胞,表皮細胞會極化形成囊腫狀, 肌肉細胞會呈纖維狀, 纖維母細胞會有各種不同於二維成長的形狀. 簡要記述(英):We demonstrate high-throughput fabrication of gelatin-based ordered cellular solids with tunable pore size and solid fraction. This process involves generating high air fraction and monodisperse liquid foam with a flow-focusing microfluidic device. The monodisperse liquid foam was further processed into open-cell solid foam, which was used as tissue-engineering scaffolds for cell culture. Three distinct cell types were cultured under these conditions and displayed appropriate physiological, morphological, and functional characteristics. Epithelial cells formed cyst-like structures and were polarized inside pores, myoblasts adopted a tubular structure and fused into myotubes, and fibroblasts exhibited wide varieties of morphologies depending on their location inside the scaffolds. These ordered cellular solids therefore make possible the study of pore-size effects on cells and the investigation of mechanical properties of microscopic foam structures. |
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(3) | 西元年:2009 研究人員(中):林耿慧 研究人員(英):LIN, KENG-HUI 研究成果名稱(中):利用微流體通道製作組織工程鷹架 研究成果名稱(英):Fabricating Scaffolds by Microfluidics 簡要記述(中):一般細胞在身體組織裡是生長在一個三維環境, 所以在三維環境下培養細胞是了解細胞如何形成組織的關鍵,在組織工程裡, 科學家製作了各種鷹架來做這件事. 但要有系統性的去了解細胞環境對細胞的影響, 鷹架的孔洞均一性就很重要.我們利用微流體通道來生產大小相同的泡泡, 這些泡泡會堆疊成有序的晶格結構, 我們用這做為樣板, 把泡泡結構變成多孔性開放的固體材料, 並做為鷹架. 在這樣的鷹架上培養細胞, 並觀察它們生長, 發現優於傳統不均勻的鷹架. 我們相信, 這個方法在三維細胞培養與了解細胞固體的力學性質的研究上有很大的應用. 簡要記述(英):Tissue cells in the body grow in a three-dimensional mesh called an extracellular matrix. It is important to create a 3D environment for the study of tissue formation. In tissue engineering, scientists fabricate all kinds of scaffold to achieve this purpose. However, to study the cell-matrix interaction systematically, it is important to have a uniform scaffold. We utitlize the microfluidic to generate monodisperse bubbles which assemble into crystalline structures. We use the foam crystal as a template and then turn it into porous gel of uniform pore size. We culture cells inside the uniform solid foam and the cell proliferate faster in comparison with the traditional non-uniform scaffold. We believe this method is very useful in the studies of 3D cell cultures and cellular solids.
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