Barcode reading of transcription factors on single DNA molecules using nanofluidic and nanoelectronic devices (AS Nano Program)
This project is to use single molecule analysis for the direct mapping (barcode reading) of TFs on genomic DNA with high spatial resolution. Our strategy is to take advantages of the confinement-induced stretching effect provided by nanofluidic channels posed on long-chain DNA polymers. Epifluorescence and FRET (fluorescence resonance energy transfer) detection scheme will be used where the spatial resolution is 1-10 nm. We also construct nm-scale nanoelectrodes which provide an excellent way to measure lateral blockage current when TF-DNA complex flows by. A set of nanoelectrode pairs will be laid along the stretching nanochannel for correlation measurement to increase the signal/noise ratio and the fidelity of the measurement.
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Figure 2. Schematic of direct mapping of protein binding sites of a coiled DNA (a), using confinement-induced stretching, (b)-(c),
POSTAL ADDRESS: 周家復老師實驗室 中央研究院物理所 北市115南港區研究院路二段128號 128, Sec. 2, Academia Rd., Nankang District, Taipei 11529, Taiwan. PHONE: 886-2-2789-6761 Office / 886-2-2789-8447/ 8908 Lab. FAX: +886-2-2651-0704 E-mail contact: cfchou@phys.sinica.edu.tw
