Nanobioscience Lab Institute of Physics Academia Sinica


P. Teerapanich, M. Pugnière, C. Henriquet, Y.L. Lin, C.F. Chou, T Leïchlé* (2017). “Nanofluidic Fluorescence Microscopy (NFM) for real-time monitoring ofprotein binding kinetics and affinity studies”, Biosensors and Bioelectronics 88, 25-33.

Rohani, B.J. Sanghavi, A. Salahi, K.T. Liao, C.F. Chou, N.S. Swami* (2017). “Frequency-selective electrokinetic enrichment of biomolecules in physiologicalmedia based on electrical double-layer polarization”, Nanoscale 9 (33), 12124-12131.

F.T. Chien, P.K. Lin, W. Chien, C.H. Hung, M.H. Yu, C.F. Chou, Y.L. Chen* (2017). “Crowding-facilitated macromolecular transport in attractive micropost arrays”, Scientific Reports 7, 1340.

K.K. Sriram, S. Nayak, S. Pengel, C.F. Chou*, A. Erbe* (2017). “10 nm deep, sub-nanoliter fluidic nanochannels on germanium for attenuated total reflection infrared (ATR-IR) spectroscopy”, Analyst 142 (2), 273-278.


R. Fernandez, B. Sanghavi, V. Farmehini, J. Chavez, J. Hagen, N. Kelley-Loughnane, C.F. Chou, N. Swami* (2016). “Aptamer-functionalized graphene-gold nanocomposites for label-free detection of dielectrophoretic-enriched neuropeptide Y”, Electrochemistry Communications 72, 144–147.

P. Teerapanich, M. Pugnière, C. Henriquet , Y. L. Lin , C.F. Chou, T. Leichle* (2016). “Nanofluidic Fluorescence Microscopy (NFM) for real-time monitoring of protein binding kinetics and affinity studies”, Biosensors and Bioelectronics,; July 2016.

A. Rohani, W. Varhue, K.T. Liao, C.F. Chou, N.S. Swami* (2016). “Nanoslit design for ion conductivity gradient enhanced dielectrophoresis for ultrafast biomarker enrichment in physiological media”, Biomicrofluidics 10, 033109.

Y.L. Lin, Y.J. Huang, P. Teerapanich, T. Leichlé, C.F. Chou* (2016). “Multiplexed immunosensing and kinetics monitoring in nanofluidic devices with highly enhanced target capture efficiency”, Biomicrofluidics, 10, 034114.

J.P. Shen and C.F. Chou* (2016). “Morphological Plasticity of Bacteria − Open Questions”, Biomicrofluidics 10, 031501.

C. Sharma, A.K. Dinda, P.D. Potdar, C.F. Chou, N.C. Mishra* (2016). “Fabrication and characterization of novel nanobiocomposite scaffold of chitosan–gelatin–alginate–hydroxyapatite for bone tissue engineering”, Materials Science & Engineering: C (Materials for Biological Applications) 64, 416–427.

B.J. Sanghavi, J.A. Moore, J.L. Chávez, J.A. Hagen, N. Kelley-Loughnane, C.F. Chou, N.S. Swami* (2016). “Aptamer-functionalized nanoparticles for surface immobilization-free electrochemical detection of cortisol in a microfluidic device”, Biosensors and Bioelectronics 78, 244–252.

P. Spuul, P.Y. Chi, C. Billottet, C.F. Chou*, E. Genot* (2016). “Microfluidic devices for the study of actin cytoskeleton in constricted environments: Evidence for podosome formation in endothelial cells exposed to a confined slit”, Methods 94, 65–74.


Y.H Su, P.C. Chiang, L.J. Cheng, C.H. Lee, N.S. Swami, C.F. Chou* (2015). “High aspect ratio nanoimprinted grooves of poly(lactic-co-glycolic acid) control the length and direction of retraction fibers during fibroblast cell division”, Biointerphases 10, 041008.

B.J. Sanghavi, W. Varhue, A. Rohani, K.T. Liao, L. Bazydlo, C.F. Chou*, N. S. Swami* (2015). “Ultrafast immunoassays by coupling dielectrophoretic biomarker enrichment on nanoslit molecular dam with electrochemical detection on graphene”, Lab Chip 15, 4563-4570.

T. Leichlé and C.F. Chou* (2015). “Biofunctionalized Nanoslit Sensors for Wash-Free and Fast Real-Time Sensing with Spatiotemporally Resolved Kinetics”, Biomicrofluidics 2015, 9, 034103 (Editor’s Picks).

J.W. Yeh, K.K. Sriram, A. Taloni, Y.L. Chen, C.F. Chou (2015). “Quantitative analysis of reptation of partially extended DNA in sub-30 nm nanoslits”, arXiv arXiv:1502.05115 [].


C.F. Chou*, P.K. Wei, Y.L. Chen (2014). “Preface to Special Topic: Selected Papers from the Advances in Microfluidics and Nanofluidics 2014 Conference in Honor of Professor Hsueh-Chia Chang's 60th Birthday”, Biomicrofluidics 8, 051901.

J.P. Shen and C.F. Chou* (2014). “Bacteria under the Physical Constraints of Periodic Micro-Nanofluidic Junctions Reveal Morphological Plasticity and Dynamic Shifting of Min Patterns”, Biomicrofluidics 2014, 8, 041103. (Fast Track article). Final list of Biomicrofluidics 2014 Best Paper Award.

K. K. Sriram, C.L. Chang, U. R. Kumar, C.F. Chou* (2014). “DNA Combing on Low-Pressure Oxygen Plasma Modified Polysilsesquioxane Substrates For Single-Molecule Studies”, Biomicrofluidics 8, 052102.

B. Sanghavi, W. Varhue, J. Chávez, C.F. Chou, N. S. Swami (2014). “Electrokinetic preconcentration and detection of neuropeptides at patterned graphene-modified electrodes in a nanochannel”, Analytical Chemistry 86, 4120–4125.

K.K. Sriram, J.W. Yeh, Y.L. Lin, Y.R. Chang, C.F. Chou* (2014). “Direct Optical Mapping of Transcription Factor Binding Sites on Field-stretched λ-DNA in Nanofluidic Devices”, Nucleic Acids Research 42, e85.

L. Lesser-Rojas, P. Ebbinghaus, G. Vasan, M.L. Chu, A. Erbe*, C.F. Chou* (2014). “Low-Copy Number Protein Detection by Electrode Nanogap-Enabled Dielectrophoretic Trapping for Surface-enhanced Raman Spectroscopy and Electronic Measurements”, Nano Letters 14, 2242–2250.

C. Lin, E.H.-L. Chen, L.Y.-L. Lee, R.L. Hsu, F.Y. Luh, L.L. Yang, C.F. Chou, L.D. Huang, C.C. Lin, R.P.-Y. Chen* (2014). “Comparison of the anti-amyloidogenic effect of O-mannosylation, O-galactosylation, and O-GalNAc glycosylation”, Carbohydrate Research 387, 46–53.

L. Lesser-Rojas, K. K. Sriram, K.T. Liao, S.C. Lai, P.C. Kuo, M.L. Chu, C.F. Chou* (2014). “Tandem array of nanoelectronic readers embedded coplanar to a fluidic nanochannel for correlated single biopolymer analysis”, Biomicrofluidics 8, 016501.

S. Gautam, C.F. Chou, A.K. Dinda, P.D. Potdar, N.C. Mishra* (2014). “Fabrication and Characterization of PCL/Gelatin/Chitosan Ternary Nanofibrous Composite Scaffold for Tissue Engineering Applications”, J. Materials Science 49, 1076-1089.

S. Gautam, C.F. Chou, A.K. Dinda, P.D. Potdar, N.C. Mishra* (2014). “Surface Modification of Nanofibrous Polycaprolactone/Gelatin Composite Scaffold by Collagen I Grafting for Skin Tissue Engineering”, Materials Science and Engineering C 34, 402–409.


A. Taloni*, J.W. Yeh, C.F. Chou* (2013). “Scaling Theory of Stretched DNA In Nanoslits”, Macromolecules 46, 7989–8002.

V. Chaurey, A. Rohani, Y.H. Su, K.T. Liao, C.F. Chou*, N.S. Swami* (2013). “Scaling down constriction-based (electrodeless) dielectrophoresis devices for trapping nanoscale bio-particles in physiological media of high-conductivity", Electrophoresis 34, 1097–1104.


1) Liao, K.-T., Tsegaye, M., Chaurey, V., Chou, C.-F., and Swami, N.S. (2012). Nano-constriction device for rapid protein preconcentration in physiological media through a balance of electrokinetic forces: Nanoanalysis. ELECTROPHORESIS 33, 1958–1966.

2) Chaurey, V., Block, F., Su, Y.-H., Chiang, P.-C., Botchwey, E., Chou, C.-F., and Swami, N.S. (2012). Nanofiber size-dependent sensitivity of fibroblast directionality to the methodology for scaffold alignment. Acta Biomaterialia 8, 3982–3990

3) K.T. Liao, C.F. Chou* (2012). “Nanoscale molecular traps and dams for ultrafast protein enrichment in high-conductivity buffers”, J. Am. Chem. Soc. (Communications)

4) P.K. Lin, C.C. Hsieh, Y.L Chen*, C.F. Chou* (2012). “Topological and Ionic Strength Influences on Confined ds-DNA Properties in Nanoslits”. Macromolecules 45 (6), 2920–2927.

5) J.W. Yeh, A. Taloni, Y.L. Chen, C.F. Chou* (2012). "Entropy-driven single moleculetug-of-war of DNA at micro-nanofluidic interfaces", Nano Letters, 12 (3), 1597–1602. DOI: 10.1021/nl2045292. (Research Highlighted by Nature,

6) T. Leïchlé, Y.L. Lin, P.C. Chiang, K.T. Liao, S.M. Hu, C.F. Chou (2012). “Biosensor-compatible encapsulation for pre-functionalized nanofluidic channels using asymmetric plasma treatment”, SENSORS AND ACTUATORS B-CHEMICAL 161, 805-810

7) V.Chaurey, C. Polanco, C.F. Chou, N.S. Swami (2012). "Floating-electrode enhanced constrictiondielectrophoresis for biomolecular trapping in media of high-conductivity", Biomicrofluidics 6, 012806.


1) C.C. Wang, Y.C. Kao, P.Y. Chi, C.W. Huang, J.Y. Lin, C.F. Chou, J.Y. Cheng and C.H. Lee (2011). "Symmetric cancer-cell filopodium growth inducedby electric-fields in a microfluidic culture chip" Lab on a Chip 11, 695-699.


1) Y.L. Chen, P.K. Lin, C.F. Chou (2010). "Generalizedforce-extension relation for worm-like chains in slit confinement", Macromolecules (Communication) 43, 10204-10207.

2) V. Chaurey, P.C. Chiang, C. Polanco, Y.H. Su, C.F. Chou, N. Swami (2010). "Interplay of Electrical Forces for Alignment of Sub-100 nm Electrospun Nanofibers on Insulator Gap Collectors", Langmuir 26, 19022-19026.

Before 2009

1) N. Swami, C.F. Chou, V. Ramamurthy, V. Chaurey (2009). "Enhancing DNA hybridization kinetics through constriction-based dielectrophoresis", Lab on a Chip 9, 3212-3220. (Highlighted on Cover)

2) J. Gu, R. Gupta, C.F. Chou, Q. Wei, F. Zenhausern (2007). “A simple polysilsesquioxane sealing of nanofluidic channels below 10 nm at room temperature”, Lab on a Chip 7, 1198-1201.

3) N. Swami, C.F. Chou, R. Terbrueggen (2005). "Two-potential electrochemical surface probe of DNA immobilization", Langmuir 21, 1937-1941.

4) L.J. Guo, X. Cheng, C.F. Chou (2004). “Fabrication of Size-Controllable Nanofluidic Channels by Nanoimprinting and Its Application for DNA Stretching”, Nano Letters 4(1) 69-73.

5)C.F. Chou, F. Zenhausern (2003). “Electrodeless Dielectrophoresis for Micro Total Analysis Systems”, IEEE Engineering for Medicine and Biology Magazine, Nov./Dec., 62-67 (invited).

6) C.Y. Chao, T.C. Pan, C.F. Chou, and J.T. Ho (2003). “Multiple Electron Diffraction and Two-Dimensional Crystalline Order in Liquid-Crystal Thin Films”, Physical Review Letters 91, 125504.

7) D. Sadler, R. Changrani, P. Roberts, C.F. Chou, F. Zenhausern (2003). “Thermal Management of BioMEMS: Temperature Control for Ceramic-based PCR and DNA Detection Devices”, IEEE Trans. on Components and Packaging Technologies 26 (2), 309-316. (Recipient of Best Paper Award of 2003)

8) C.F. Chou, J.O. Tegenfeldt, O. Bakajin, S.S. Chan, E.C. Cox, N. Darnton, T.A.J. Duke, R.H. Austin (2002). “Electrodeless Dielectrophoresis of Single and Double Stranded DNA”, Biophysical Journal 83, 2170-2179.

9) C.F. Chou, R. Changrani, P. Roberts, D. Sadler, S. Lin, A. Mulholland, N. Swami, R. Terbrueggen, F. Zenhausern (2002). “A Miniaturized Cyclic PCR Device-Modeling and Experiments”, Microelectronic Engineering 61-62, 921-925.

10) O. Bakajin, T. A. J. Duke, J. Tegenfeldt, C. F. Chou, S. S. Chan, R. H. 18)  Austin and E. C. Cox (2001). “Separation of 100 kilobase DNA molecules in 10 seconds,” Analytical Chemistry 73, 6053-6056 (accelerated article).  

11) J. O. Tegenfeldt, O. Bakajin, C. F. Chou, S. S. Chan, R. H. Austin, W. Fann, L. Liou, E. Chan, T. Duke, and E. C. Cox (2001). “Near-Field Scanner for Moving Molecules”, Physical Review Letters 86, 1378-1381 (Physics Focus on Physics Today).  

12) C.F. Chou, R.H. Austin, O. Bakajin, J.O. Tegenfeldt, J.A. Castelino, S.S. Chan, E.C. Cox, H.G. Craighead, N. Darton, T.A.J. Duke, J. Han, S.W. Turner (2000). “Sorting Biomolecules with microdevices”, Electrophoresis 21, 81-90 (invited).

13) C.F. Chou, O. Bakajin, S.W. Turner, T. Duke, S.S. Chan, R.H. Austin, E.C. Cox, and H.G. Craighead (1999). “Sorting by Diffusion: an Asymmetric Obstacle Course for continuous molecular separation”, Proceedings of National Academy of Sciences, USA, 96, 13762-13765. 

14) C.F. Chou, A.J. Jin, S.W. Hui, C.C. Huang, and J.T. Ho (1998). “Multiple-Step Melting in Two-Dimensional Hexatic Liquid-Crystal Films”, Science 280, 1424-1426. 

15) O.B. Bakajin, T.A.J. Duke, C.F. Chou, S.S. Chan, R.H. Austin, and E.C. Cox (1998). “Electrohydrodynamic Stretching of DNA in Confined Environments”, Physical Review Letters 80, 2737-2740.

16) C.Y. Chao, S.W. Hui, J.E. Maclennan, C.F. Chou, and J.T. Ho (1997). “Surface-Freezing Transitions and Novel Tilted Hexatic Phases in Smectic Liquid-Crystal Films”, Physical Review Letters 78, 2581-2584. 

17) A.J. Jin, C.C. Huang, C.F. Chou, C.Y. Chao, and J.T. Ho (1996). “Studies of Surface-Induced Layer-by-Layer Two-Stage Phase Transformations”, Modern Physics Letters B 10, 765-770.

18) C.Y. Chao, C.F. Chou, J.T. Ho, S.W. Hui, A.J. Jin, and C.C. Huang (1996). “Nature of Layer-by-Layer Freezing in Free-Standing 4O.8 Films”, Physical Review Letters 77, 2750-2753. 

19) C.F. Chou, J.T. Ho, S.W. Hui, and V. Surendranath (1996). “Scaling of 6n-Fold Bond-Orientational Order Parameters in a Hexatic Liquid-Crystal Thin Film”, Physical Review Letters 76, 4556-4559. 

20) A.J. Jin, M. Veum, T. Stoebe, C.F. Chou, J.T. Ho, S.W. Hui, V. Surendranath, and C.C. Huang (1995). “Calorimetric and Structural Characterization of Thin Liquid-Crystal Films Exhibiting the Smectic-A–Hexatic-B–Crystal-B Transitions”, Physical Review Letters 74, 4863-4866.

POSTAL ADDRESS: 周家復老師實驗室 中央研究院物理所 北市115南港區研究院路二段128號 128, Sec. 2, Academia Rd., Nankang District, Taipei 11529, Taiwan. PHONE: 886-2-2789-6761 Office / 886-2-2789-8907/ 8354 Lab. FAX: +886-2-2651-0704 E-mail contact: