Dragonfly200 confocal microscope
【儀器管理員 Administrators】
- 陳易詩 Chen, Yi-Shih / as0191533[at]gate.sinica.edu.tw / 02-2789-8978 (0911-663-539)
- 沈介磐 Shen, Jie-Pan / jpshen[at]phys.sinica.edu.tw / 02-2789-8907
- 林耿慧 Lin, Keng-hui / khlin[at]phys.sinica.edu.tw / 02-2789-6763
【特殊安全規則 Special safety notices and regulations】
使用者安全與儀器保全 User and instrumentation safety
- 確實填寫紀錄本，未確實填寫者停權兩周。儀器若有任何問題，請立即向管理員反映。
Fill out the logbook accurately; failure to do so can lead to the penality of two-week account suspension. Inform administrators immediately of any apparatus issues. - 若有需要(例如使用 CO2)，請啟動氧氣偵測器，監控氧氣濃度。請詳讀使用 CO2 安全規定。
Turn on the O2 detector to monitor the O2 level if needed (e.g., CO2 is in use). Please read the Safety Protocol for CO2 usage. - 總是從較短的曝光時間開始，避免過度曝光，損壞昂貴的相機。
Always start with short camera exposure time to prevent overexposure and damage to the expensive cameras. - 請勿更改任何電腦、硬體及軟體的設定。實驗時把數據存放在指定資料夾內，並在實驗後從電腦移除。所有數據會在周五或必要時刪除。
Do not modify any setup parameter value of the computer, hardware, and software. Save data in a designated folder during experiment and remove data from the computer after experiment. All data will be deleted on Fridays or whenever necessary. - 油鏡每次使用完畢必需清潔，並檢查確認其它物鏡及載物台面沒有被鏡油汙染。合格使用者應該熟悉清潔物鏡與移除油汙的標準作業程序。
If immersion oil is used, clean the objectives properly and immediately after experiment; inspect the other objectives and the stage for possible contamination by oil. Qualified users should be familiar with the SOPs for cleaning objectives and removing oil contamination. - 如需更換物鏡，請洽管理員。
Please contact administrators for objective replacement or installation. - 實驗室備有訪客用室內鞋，經常使用儀器者應自帶實驗室用鞋。
Indoor shoes for visitors are provided. Frequent users should bring their own laboratory shoes.
【儀器重點規格 Main specifications】
Constact admininstrators for detailed specs.
- 名稱 Name: 高速轉盤式共軛焦顯微鏡 High-speed spinning-disk confocal microscope
- 廠牌/型號 Manufacturer/Model: Andor/Dragonfly200
- 顯微鏡 Microscope: Olympus IX83; Apochromat objectives 10X, 20X, 40X, 60XSiO, 100XO; bright field and fluorescence
- 共軛焦 Confocal: dual pinhole; lasers: 405nm, 488nm, 561nm, 637nm; multiple bandpass filters and dichroic splitters (contact administrators for details)
- 軟體 Software: Fusion and Imaris
- 相機1 Camera 1: Andor Zyla sCMOS (2048x2048)
- 相機2 Camera 2: Andor iXon Life 888 EMCCD (1024x1024)
- XYZ 電動載物台 XYZ Automated Stage: ASI PZ-2500 XYZ Automated Stage with Piezo Z-axis Top Plate
- 活細胞培養裝置 Incubator: OKOLAB, electrically heated stage top chamber(H301-K-FRAME), #1 1”x3”chamber slide + #2 35mm Petri dishes holder(GS35-M), temperature, CO2, humidity controllers, air pump
【使用者資格與教育訓練 User qualification and training】
- 合格使用者須通過管理員認可的教育訓練，包括現場教學與實習。教育訓練會定期實施或依需求辦理。只有合格使用者才能進入實驗室並預約使用儀器。
Qualified users must pass the training certified by the administrators. The training includes on-site tutorial and practice sessions and will be offered regularly or on demand. Only qualified users can gain access to the lab and reserve the instrument. - 預備知識：參加教育訓練的使用者需已熟悉光學顯微鏡的操作保養以及共軛焦顯微技術的原理(Wiki)。
Prerequisites: Users should be familiar with the practical operation and maintenance of optical microscopes and the principle of confocal microscopy(Wiki). - 教育訓練資源與教材 Training resources and materials: Confocal(Nikon), Confocal(Andor), Cleaning oil immersion objectives(Olympus), BSSF Dragonfly 200 quick user manual, FUSION Software Guide, the Safety Protocol for CO2 usage.
【預約 Reservation】
【收費 Charge】
- No charge yet.
【公共儀器違規罰則說明Rules and Penalties for Misuse of Public Instruments】
為確保儀器資源公平使用與維護儀器運作品質，請所有使用者遵守以下規範。違規情形與處理辦法如下：
To ensure fair use of shared equipment and maintain operational quality, all users must follow the rules below. Violations and penalties are as follows:
| 違規項目Violation | 處理方式Penalty |
|---|---|
| 預約後未使用且未事先取消 No-show without prior |
|
| 未依規定登記使用紀錄 Failure to log usage as required |
|
| 操作不當造成儀器損壞 Damage to equipment due to improper operation |
依損壞程度賠償並停權 1 個月至 3 個月 Compensation based on damage level, plus 1–3 month suspension |
| 使用他人帳號預約或操作 Using another user’s account to book or operate |
雙方皆停權 1 個月 Both parties suspended for 1 month |
注意事項 / Notes:
1. 若有特殊情況，請事先告知管理員。
If special circumstances occur, notify the administrator in advance.
2. 停權期間不得以任何方式使用儀器或代他人操作。
During suspension, users may not use equipment or operate on behalf of others.
3. 規則將視情況調整，最新版本以公告為準。
Rules may be adjusted; the latest version in the official announcement prevails.
4. 臨時取消預約者請在使用者群組告知其他使用者，若是無法取消線上預約可以請管理者協助。
Users who need to cancel a reservation at the last minute should notify other users in the user group. If you are unable to cancel the reservation online, you may request assistance from the administrator.
5. 如果你想預約時間與其他使用者有衝突時，可以自行與其他使用者溝通或是經由管理者協助溝通。
If your desired reservation time conflicts with another user’s booking, you may communicate directly with the other user or seek assistance from the administrator to coordinate.