Introduction to Nano-biophotonics
Instructor: Chia-Fu Chou ©P®a´_
Time: Wed. 9:10-12:00
Classroom: be announced
Office hours: Mon. 10-12 @ P612, IoP
Introdution:¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@¡@
In this course, we will review the basics and recent developments of nanobiophotonics, an emerging field which is cross-displinary among nanoscience and nanotechnology, biology, and photonics. The course is comprised of lectures from several special topics given by experts in these areas, including micro/nanofluidics, single molecule biophysics, DNA microarray, polymer brush, optical tweezers, near-field optics, plasmonics (SPR, SERS), advanced bioimaging techniques (fluorescence, confocal, CARS, TIRFM, FRET, higher harmonics, multiphoton, X-ray nanoimaging, super-high resolution imaging, etc.), image analysis and lab tours. Grading will be based on term project presentation and report.
Grade evaluation:
Term project presentation¡V50%
Written report (no more than 10 pages, due 1/12/09)¡V50%
Attendance:
Students will need to sign the attendance sheet for each class. Any student who has 2 no-shows will be failed from the course. The instructor should be informed in advance if medical absence or other emergency matters occurred.
Speakers and topics to be covered:
Chang, Wei-Hau ³¹¬°µq(1) ¡V Single Molecule FRET
Cheng, Ji-Yen ¾Gл¨¥ (1) ¡V DNA microarrays-fabrication, detection, and analysis
Chou, Chia-Fu ©P®a´_ (7) ¡V Introduction, micro/nanofluidics, SM biophys, bioimaging, Fluorescence microscopy, TIRFM, Term project assignment & evaluation
Gukassyan, Vladimir (1) (YMU) ¡V Time-resolved fluorescence microscopy (FLIM, FCS, FRET, TCSPC)
Hwu, Yeu-Kuang J¦t¥ú(1) ¡V X-ray nanoimaging
Juan, Wen-Tau ¨¿¤å·Ê(1) Polymer brush, optical tweezers
Lee, Chau-Hwang §õ¶W·× (1) ¡V Confocal, NIWOP-cell membrane imaging, image processing
Tang, Jau ´ö´Â·u(1) ¡V Studies of single molecules using confocal microscopy; photophysical properties of quantum dots; studies of electron and energy transfer reactions; single-enzyme catalytic reactions; ultrafast electron diffraction
Wei, Pei-Kuen ÃQ°ö©[ (1) ¡V Near-field optics/SNOM, SERS, Nanoscale optical control and biosensing
Yang, Wei-Yuan ·¨ºû¤¸(1)¡V CARS Microscopy
Class Schedule for Fall Semester, 2008
Week |
Wednesday |
9:10-12:00 |
Week |
Wednesday
|
9:10-12:00 |
1 |
97/09/24 |
Lee, Chau-Hwang |
9 |
97/11/19 |
Tang, Jau |
2 |
97/10/01 |
Chou, Chia-Fu |
10 |
97/11/26 |
Chang, Wei-Hau |
3 |
97/10/08 |
Chou, Chia-Fu |
11 |
97/12/03 |
Yang, Wei-Yuan |
4 |
97/10/15 |
Wei, Pei-Kuen |
12 |
97/12/10 |
Cheng, Ji-Yen |
5 |
97/10/22 |
Gukassyan, Vladimir |
13 |
97/12/17 |
Student presentation
Chou, Chia-Fu
|
6 |
97/10/29 |
Hwu, Yeu-Kuang |
14 |
97/12/24 |
Student presentation
Chou, Chia-Fu
|
7 |
97/11/05 |
Term project ssignment / Lab tour
Chou, Chia-Fu
|
15 |
97/12/31 |
Student presentation
Chou, Chia-Fu |
8 |
97/11/12 |
Juan, Wen-Tau |
16 |
98/01/07 |
Student presentation
Chou, Chia-Fu |
Reference:
- Optical microscopy in general- http://micro.magnet.fsu.edu/
- Stefan A. Maier: Plasmonics: Fundamentals and Applications (Springer, 2007)
- Lukas Novotny and Bert Hecht: Principles of Nano-Optics (Cambridge University Press, 2006)
- Joseph R. Lakowicz: Principles of Fluorescence Spectroscopy (3rd Ed., Springer, 2006)
- Alastair Smith, Christopher Gell, and David Brockwell: Handbook of Single Molecule Fluorescence Spectroscopy (Oxford University Press, 2006)
- Katrin Kneipp, Martin Moskovits, and Harald Kneipp: Surface-Enhanced Raman Scattering: Physics and Applications (Springer, 2006)
- Wolfgang Becker: Advanced Time-Correlated Single Photon Counting Techniques (Springer Series in Chemical Physics, 2005)
- Christof M. Niemeyer and Chad A. Mirkin (Editors): Nanobiotechnology: Concepts, Applications and Perspectives (Hardcover, Wiley-VCH, 2004)
- Roland Glaser: Biophysics (Springer, 2004)
- Paras N. Prasad: Introduction to Biophotonics (Wiley-Interscience, 2003)
- West JL, Halas NJ: Engineered nanomaterials for biophotonics applications: Improving sensing, imaging, and therapeutics. Annual Review of Biomedical Engineering 5: 285-292 2003.
- H. Berg, The Random Walk in Biology, Princeton University Press, 1993.
Term project topics: (term project report due 1/12/09)
Topics:
- Precise nanometer localization analysis for individual fluorescent probes.
Biophys. J. 82: 2775-2783, 2002. (Paul Cardenas)
- Single-Molecule Analysis 2: Single-Molecule Fluorescence Resonance Energy
Transfer. TJ Ha. METHODS 25, 78¡V86, 2001. (Fei Pei)
- FRET imaging. Nature Biotech. 21: 1387-1395, 2003. (Li-Wei Huang, Chih-
Ning Chen)
- FRET or no FRET: A quantitative comparison. Biophys. J. 84: 3992¡V4010,
2003. (Mohamed Abbass, Mohamad Yaseen)
- Single-quantum-dot-based DNA nanosensor. Nature Materials 4, 826-831,
2005. (Chia-Jong Liu, Meng-Lin Tsai)
- Resolution scaling in STED microscopy. Harke et al., OPTICS EXPRESS 16,
4154-4162, 2008. (Kumar Sharma)
- Biolabeling with nanoparticles (gold NPs, QDs, and nanodiamonds) (Chen-
Hon Nee, Huai-Yi Chen)
- CNT biosenor: Cellular uptake of functionalized carbon nanotubes is
independent of functional group and cell type. Nature Nanotechnology, 2,
108¡V113, 2007. (Bhagava Billa)
- Photo-nanobiotechnology: Quantum dots and nanoparticles for
photodynamic and radiation therapies of cancer. Advanced Drug Delivery
Reviews 2008 (in press); Trends in Biotechnology Vol.26 No.11 (in press,
doi:10.1016/j.tibtech.2008.07.007). (Tony Rajan)
97/12/17 (W) |
1. Abbass/Yaseen
2. Pei.
3. Toney
|
97/12/23 (Tu.) |
1. Paul
2. Li-Wei/Chih-Ning
|
97/12/24 (W) |
1. Chia-Jong/Meng-Lin
2. Chen-Hon/Huai-Yi
|
97/12/31 (W) |
|
Classnote:
2008 / 9 / 24 |
Modern Optical Microscopy |
2008 / 10 / 15 |
Surface Plasmon_1
Surface Plasmon_2 |
2008 / 10 / 22 |
Time-Resolved Mocroscopy |
2008 / 11 / 12 |
Optical Tweezers |
2008 / 11 / 19 |
Studies of light-induced stochastic and coherent
processes in nano-structured materials
using single-molecule techniques and
femtosecond time-resolved electron diffraction |