2025/08/18(Mon) 10:30 -12:00 五樓第一會議室 5F, 1st Meeting Room
Title
Frontier Biomedical applications with TPS 13A SWAXS
Speaker
鄭有舜博士 (財團法人國家同步輻射研究中心科學研究組軟物質科學小組) Dr. U-Ser Jeng (National Synchrotron Radiation Research Center (NSRRC))Abstract
The 13A biological small- and wide-angle X-ray scattering (SWAXS) beamline at the Taiwan Photon Source (TPS) is equipped with dual in-vacuum detectors and an integrated online size-exclusion chromatography (SEC) system, enabling the investigation of biomolecular structures and compositions in physiological solution across a broad range of length scales. When coupled with molecular dynamics (MD) simulations, this SWAXS-MD approach facilitates the reconstruction of all-atom models of biomolecules, capturing their domain interfaces and hydration shell structures in detail. Using this methodology, we showcase several successful biomedical applications: elucidating binding structures and efficacies of protein-drug complexes in solution, distinguishing serum protein conformations in a series of medicated serum samples from a lupus nephritis (LN) patient, and characterizing drug loading and trigger-release structural changes in liposomes and copolymer micelles, and gene-loaded lipid nanoparticles. In conjunction with laboratory-based SEC-MALS-DLS measurements, the TPS SEC-SWAXS platform provides molecular-level characterizations in biomedical research and industrial applications, and offers potential for early diagnosis of serum protein relevant diseases, as well as rapid structural controls of nanoscale drug delivery systems.
Poster
Language
演講語言 (Language): in English