2016/12/26(Mon) 11:00 -12:00 視訊會議室(圖書館) 3F, Video Conference Room(Library)
Title
Deciphering the role of ionic liquids in the structure and stability of biomolecules
Speaker
Prof. Pannuru Venkatesu (Department of Chemistry, University of Delhi, Delhi)Abstract
We have investigated the biomolecular interactions and related associated structural changes of α-chymotrypsin (CT) with new ILs by using several biophysical techniques including circular dichroism (CD) and fluorescence. The ILs studied in the present study includes diethylammonium dihydrogen phosphate [(CH3CH2)2NH][H2PO4] (DEAP), diethylammonium hydrogen sulfate [(CH3CH2)2NH][HSO4] (DEAS), triethylammonium dihydrogen phosphate [(CH3CH2)3NH][H2PO4] (TEAP) and triethylammonium hydrogen sulfate [(CH3CH2)3NH][HSO4] (TEAS). We observed that all ILs have dominant contribution to the stabilization of the native structure of the CT. Furthermore, the results reveal that phosphate anions of ILs are strong stabilizers and acted as effective refolding enhancers for thermally denatured enzyme structure, whereas the enzyme was not refolded in the sulfate anions of ILs. These findings suggest a new generation of enzyme stabilizers that can be applied to other protein folding studies and biological systems.
Language
演講語言 (Language): in English