周家復 / 研究員

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連絡資訊

學歷

  • 美國紐約州立大學水牛城分校 物理學博士
  • 國立清華大學物理系學士

秘書

周泳恩 / 886-2-2789-8364

研究興趣

生物物理、生醫光電、奈米生技、微奈米流體、液晶物理 ;

經歷

  • 2007- 合聘研究員, 基因體研究中心, 中央研究院
  • 2006- 合聘研究員, 應用科學研究中心, 中央研究院
  • 2005- 合聘副教授, 生醫工程系, 亞利桑那州立大學
  • 2003-2005- 副教授暨首要研究員暨共同創辦人,應用奈米生醫中心, 生物設計研究院,亞利桑那州立大學
  • 2000-2002 首要科學家, 微工藝研究室, 摩托羅拉實驗室
  • 1997-2000 NIH 博士後研究員, 物理系及分子生物系, 普林斯頓大學

學術著作

期刊論文

  • [1]     A. Rohani, B. J. Sanghavi, A. Salahi, K. T. Liao, C. F. Chou, N. S. Swami*, 2017, “Frequency-selective electrokinetic enrichment of biomolecules in physiological media based on electrical double-layer polarization”, NANOSCALE, 9, 12124-12131. (SCI) (IF: 7.76; SCI ranking: 11%,8.5%,14.5%,8.3%)

  • [2]     F. T. Chien, P. K. Lin, W. Chien, C. H. Hung, M. H. Yu, C. F. Chou, Y. L. Chen*, 2017, “Crowding-facilitated macromolecular transport in attractive micropost arrays”, SCIENTIFIC REPORTS, 7, 1340. (SCI) (IF: 5.228; SCI ranking: 11.1%)

  • [3]     P. Teerapanich, M. Pugnière, C. Henriquet , Y. L. Lin , C. F. Chou, T. Leichle*, 2017, “Nanofluidic Fluorescence Microscopy (NFM) for real-time monitoring of protein binding kinetics and affinity studies”, BIOSENSORS & BIOELECTRONICS, 88, 25-33. (SCI) (IF: 7.476; SCI ranking: 4%,15.7%,8.3%,3.7%,6.2%)

  • [4]     K. K. Sriram, S. Nayak, C.F. Chou*, A. Erbe*, 2017, “10-nm Deep, Sub-Nanoliter Fluidic Nanochannels on Germanium for Attenuated Total Reflection Infrared (ATR-IR) Spectroscopy”, ANALYST, 142, 273-278. (SCI) (IF: 4.033; SCI ranking: 13.3%)

  • [5]     R. Fernandez, B. Sanghavi, V. Farmehini, J. Chavez, J. Hagen, N. Kelley-Loughnane, C.F. Chou, N. Swami*, 2016, “Aptamer-functionalized graphene-gold nanocomposites for label-free detection of dielectrophoretic-enriched neuropeptide Y”, ELECTROCHEMISTRY COMMUNICATIONS, 72, 144–147. (SCI) (IF: 4.569; SCI ranking: 18.5%)

  • [6]     C. Sharma, A.K. Dinda, P.D. Potdar, C.F. Chou, N.C. Mishra *, 2016, “Fabrication and characterization of novel nanobiocomposite scaffold of chitosan–gelatin–alginate–hydroxyapatite for bone tissue engineering”, Materials Science & Engineering C-Materials for Biological Applications, 64, 416–427. (SCI) (IF: 3.42; SCI ranking: 36.4%)

  • [7]     J.P. Shen and C.F. Chou* , 2016, “Morphological Plasticity of Bacteria − Open Questions”, Biomicrofluidics, 10(3), 031501. (SCI) (IF: 2.708; SCI ranking: 40.3%,20%,42.2%,41.6%)

  • [8]     Y.L. Lin, Y.J. Huang, P. Teerapanich, T. Leichlé, C.F. Chou* , 2016, “Multiplexed immunosensing and kinetics monitoring in nanofluidic devices with highly enhanced target capture efficiency”, Biomicrofluidics, 10(3), 034114. (SCI) (IF: 2.708; SCI ranking: 40.3%,20%,42.2%,41.6%)

  • [9]     A. Rohani, W. Varhue, K.T. Liao, C.F. Chou, N.S. Swami* , 2016, “Nanoslit design for ion conductivity gradient enhanced dielectrophoresis for ultrafast biomarker enrichment in physiological media”, Biomicrofluidics, 10, 033109. (SCI) (IF: 2.708; SCI ranking: 40.3%,20%,42.2%,41.6%)

  • [10]     B.J. Sanghavi, J.A. Moore, J.L. Chávez, J.A. Hagen, N. Kelley-Loughnane, C.F. Chou, N.S. Swami, 2016, “Aptamer-functionalized nanoparticles for surface immobilization-free electrochemical detection of cortisol in a microfluidic device”, BIOSENSORS & BIOELECTRONICS, 78, 244–252. (SCI) (IF: 7.476; SCI ranking: 4%,15.7%,8.3%,3.7%,6.2%)

  • [11]     P. Spuul, P.Y. Chi, C. Billottet, C.F. Chou*, E. Genot* , 2016, “Microfluidic devices for the study of actin cytoskeleton in constricted environments: Evidence for podosome formation in endothelial cells exposed to a confined slit”, METHODS, 94, 65–74. (SCI) (IF: 3.503; SCI ranking: 23.4%,34.3%)

  • [12]     Y.H Su, P.C. Chiang, L.J. Cheng, C.H. Lee, N.S. Swami*, C.F. Chou* , 2015, “High aspect ratio nanoimprinted grooves of poly(lactic-co-glycolic acid) control the length and direction of retraction fibers during fibroblast cell division”, Biointerphases, 10, 041008. (SCI) (IF: 2.105; SCI ranking: 57.6%,58.3%)

  • [13]     B.J. Sanghavi, W. Varhue, A. Rohani, K.T. Liao, L. Bazydlo, C.F. Chou*, N. S. Swami* , 2015, “Ultrafast immunoassays by coupling dielectrophoretic biomarker enrichment on nanoslit molecular dam with electrochemical detection on graphene”, Lab Chip, 15, 4563-4570. (SCI) (IF: 5.586; SCI ranking: 10.4%,15.3%,22.9%)

  • [14]     T. Leichlé and C.F. Chou* , 2015, “Biofunctionalized Nanoslit Sensors for Wash-Free and Fast Real-Time Sensing with Spatiotemporally Resolved Kinetics”, Biomicrofluidics, 9, 034103. (SCI) (IF: 2.708; SCI ranking: 40.3%,20%,42.2%,41.6%)

  • [15]     C. F. Chou*, P. K. Wei, and Y. L. Chen , 2014, “Preface to Special Topic: Selected Papers from the Advances in Microfluidics and Nanofluidics 2014 Conference in Honor of Professor Hsueh-Chia Chang's 60th Birthday”, Biomicrofluidics, 8, 051901. (SCI) (IF: 2.708; SCI ranking: 40.3%,20%,42.2%,41.6%)

  • [16]     K. K. Sriram, C.L. Chang, U. R. Kumar, C.F. Chou*, 2014, “DNA Combing on Low-Pressure Oxygen Plasma Modified Polysilsesquioxane Substrates For Single-Molecule Studies”, Biomicrofluidics, 8, 052102. (SCI) (IF: 2.708; SCI ranking: 40.3%,20%,42.2%,41.6%)

  • [17]     J. P. Shen, C. F. Chou* , 2014, “Bacteria under the Physical Constraints of Periodic Micro-Nanofluidic Junctions Reveal Morphological Plasticity and Dynamic Shifting of Min Patterns”, Biomicrofluidics, 8, 041103 (Fast Track Article). (SCI) (IF: 2.708; SCI ranking: 40.3%,20%,42.2%,41.6%)

  • [18]     K.K. Sriram, J.W. Yeh, Y.L. Lin, Y.R. Chang, C.F. Chou*, 2014, “Direct Optical Mapping of Transcription Factor Binding Sites on Field-stretched λ-DNA in Nanofluidic Devices”, NUCLEIC ACIDS RESEARCH, 42, e85. (SCI) (IF: 9.202; SCI ranking: 6.2%)

  • [19]     B. Sanghavi, W. Varhue, J. Chávez, C.F. Chou, N. S. Swami* , 2014, “Electrokinetic preconcentration and detection of neuropeptides at patterned graphene-modified electrodes in a nanochannel”, ANALYTICAL CHEMISTRY, 86 (9), 4120–4125. (SCI) (IF: 5.886; SCI ranking: 5.3%)

  • [20]     C. Lin, E. H.-L. Chen, L. Y.-L. Lee, R. L. Hsu, F. Y. Luh, L. L. Yang, C. F. Chou, L. D. Huang, C. C. Lin, R. P.-Y. Chen*, 2014, “Comparison of the anti-amyloidogenic effect of O-mannosylation, O-galactosylation, and O-GalNAc glycosylation”, CARBOHYDRATE RESEARCH, 387, 46–53. (SCI) (IF: 1.817; SCI ranking: 57.6%,75.1%,40.3%)

  • [21]     S. Gautam, C. F. Chou, A. K. Dinda, P. D. Potdar, N. C. Mishra* , 2014, “Fabrication and Characterization of PCL/Gelatin/Chitosan Ternary Nanofibrous Composite Scaffold for Tissue Engineering Applications”, JOURNAL OF MATERIALS SCIENCE, 49, 1076–1089. (SCI) (IF: 2.302; SCI ranking: 30.3%)

  • [22]     L. Lesser-Rojas, P. Ebbinghaus, G. Vasan, M. L. Chu, A. Erbe*, C. F. Chou*, 2014, “Low-Copy Number Protein Detection by Electrode Nanogap-Enabled Dielectrophoretic Trapping forSurface-enhanced Raman Spectroscopy and Electronic Measurements”, NANO LETTERS, 14(5), 2242–2250. (SCI) (IF: 13.779; SCI ranking: 4.8%,4.3%,3%,2.8%,9%,3.6%)

  • [23]     S. Gautam, C. F. Chou, A. K. Dinda, P. D. Potdar, N. C. Mishra*, 2014, “Surface Modification of Nanofibrous Polycaprolactone/Gelatin Composite Scaffold by Collagen I Grafting for Skin Tissue Engineering”, Materials Science & Engineering C-Materials for Biological Applications, 34, 402–409. (SCI) (IF: 3.42; SCI ranking: 36.4%)

  • [24]     L. Lesser-Rojas, K. K. Sriram, K.T. Liao, S.C. Lai, P.C. Kuo, M.L. Chu, C.F. Chou*, 2014, “Tandem array of nanoelectronic readers embedded coplanar to a fluidic nanochannel for correlated single biopolymer analysis”, Biomicrofluidics, 8, 016501. (SCI) (IF: 2.708; SCI ranking: 40.3%,20%,42.2%,41.6%)

  • [25]     A. Taloni*, J. W. Yeh, C. F. Chou* , 2013, “Scaling Theory of Stretched DNA In Nanoslits”, MACROMOLECULES, 46 (19), 7989–8002. (SCI) (IF: 5.554; SCI ranking: 8.2%)

  • [26]     V. Chaurey, A. Rohani, Y.H. Su, K.T. Liao, C.F. Chou*, N.S. Swami* , 2013, “Scaling down constriction-based (electrodeless) dielectrophoresis devices for trapping nanoscale bio-particles in physiological media of high-conductivity”, ELECTROPHORESIS, 34, 1097–1104.. (SCI) (IF: 2.482; SCI ranking: 34.7%,49.4%)

  • [27]     V. Chaurey, F. Block, Y.H. Su, P.C. Chiang, E. Botchwey, C.F. Chou, N.S. Swami*, 2012, “Nanofiber size-dependent sensitivity of fibroblast directionality to the methodology for scaffold alignment”, Acta Biomaterialia, 8, 3982–3990. (SCI) (IF: 6.008; SCI ranking: 6.1%,3.9%)

  • [28]     Kuo-Tang Liao, Chia-Fu Chou*, 2012, “Nanoscale molecular traps and dams for ultrafast protein enrichment in high-conductivity buffers (Featured in JACS Spotlights)”, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 134 (21), 8742−8745. (SCI) (IF: 13.038; SCI ranking: 6.1%)

  • [29]     Jia-Wei Yeh, Alessandro Taloni, Yeng-Long Chen, Chia-Fu Chou*, 2012, “Entropy-driven single molecule tug-of-war of DNA at micro-nanofluidic interfaces (Research Highlighted by Nature)”, NANO LETTERS, 12 (3), 1597–1602. (SCI) (IF: 13.779; SCI ranking: 4.8%,4.3%,3%,2.8%,9%,3.6%)

  • [30]     T. Leïchlé, Y.L. Lin, P.C. Chiang, K.T. Liao, S.M. Hu, C.F. Chou* , 2012, “Biosensor-compatible encapsulation for pre-functionalized nanofluidic channels using asymmetric plasma treatment”, SENSORS AND ACTUATORS B-CHEMICAL, 161, 805–810. (SCI) (IF: 4.758; SCI ranking: 9.3%,3.6%,14.8%)

  • [31]     P.K. Lin, C.C. Hsieh, Y.L Chen*, C.F. Chou* , 2012, “Effects of Topology and Ionic Strength on Double-Stranded DNA Confined in Nanoslits”, MACROMOLECULES, 45(6), 2920–2927. (SCI) (IF: 5.554; SCI ranking: 8.2%)

  • [32]     V. Chaurey, C. Polanco, C.F. Chou*, N.S. Swami*, 2012, “Floating-electrode enhanced constriction dielectrophoresis for biomolecular trapping in media of high-conductivity”, Biomicrofluidics, 6, 012806. (SCI) (IF: 2.708; SCI ranking: 40.3%,20%,42.2%,41.6%)

  • [33]     V. Chaurey, K.T. Liao, C. Polanco, C.F. Chou*, N.S. Swami*, 2012, “Nano-constriction device for rapid protein pre-concentration through balance of electrokinetic forces”, ELECTROPHORESIS, 33, 1958-1966. (SCI) (IF: 2.482; SCI ranking: 34.7%,49.4%)

  • [34]     C.C. Wang, Y.C. Kao, P.Y. Chi, C.W. Huang, J.Y. Lin, C.F. Chou, J.Y. Cheng*, C.H. Lee, 2011, “Asymmetric cancer-cell filopodium growth induced by electric-fields in a microfluidic culture chip”, LAB ON A CHIP, 11, 695-699. (SCI) (IF: 5.586; SCI ranking: 10.4%,15.3%,22.9%)

  • [35]     Y.L. Chen, P. K. Lin, C.F. Chou , 2010, “Generalized force-extension relation for worm-like chains in slit confinement”, MACROMOLECULES, 43, 10204–10207. (SCI) (IF: 5.554; SCI ranking: 8.2%)

  • [36]     V. Chaurey, P.C. Chiang, C. Polanco, Y.H. Su, C.F Chou*, N. Swami*, 2010, “Interplay of Electrical Forces for Alignment of Sub-100 nm Electrospun Nanofibers on Insulator Gap Collectors”, LANGMUIR, 26, 19022–19026. (SCI) (IF: 3.993; SCI ranking: 21.5%,25%,17%)

  • [37]     N. Swami, C. F. Chou*, V. Ramamurthy, V. Chaurey , 2009, “Enhancing DNA hybridization kinetics through constriction-based dielectrophoresis”, LAB ON A CHIP, 9, 3212. (SCI) (IF: 5.586; SCI ranking: 10.4%,15.3%,22.9%)

  • [38]     J. Gu, R. Gupta, C.F. Chou, Q. Wei, F. Zenhausern, 2007, “A simple polysilsesquioxane sealing of nanofluidic channels below 10 nm at room temperature”, LAB ON A CHIP, 7, 1198. (SCI) (IF: 5.586; SCI ranking: 10.4%,15.3%,22.9%)

  • [39]     N. Swami, C.F. Chou, R. Terbrueggen , 2005, “Two-potential electrochemical surface probe of DNA immobilization”, LANGMUIR, 21, 1937. (SCI) (IF: 3.993; SCI ranking: 21.5%,25%,17%)

  • [40]     L.J. Guo, X. Cheng, C.F. Chou* , 2004, “Fabrication of Size-Controllable Nanofluidic Channels by Nanoimprinting and Its Application for DNA Stretching”, NANO LETTERS, 4, 69-73. (SCI) (IF: 13.779; SCI ranking: 4.8%,4.3%,3%,2.8%,9%,3.6%)

  • [41]     C.F. Chou*, F. Zenhausern , 2003, “Electrodeless Dielectrophoresis for Micro Total Analysis Systems”, IEEE ENGINEERING IN MEDICINE AND BIOLOGY MAGAZINE, 22, 62-67.

  • [42]     C.Y. Chao, T.C. Pan, C.F. Chou, J.T. Ho , 2003, “Multiple Electron Diffraction and Two-Dimensional Crystalline Order in Liquid-Crystal Thin Films”, PHYSICAL REVIEW LETTERS, 91, 125504. (SCI) (IF: 7.645; SCI ranking: 7.6%)

  • [43]     D. Sadler, R. Changrani, P. Roberts, C.F. Chou, F. Zenhausern , 2003, “Thermal Management of BioMEMS: Temperature Control for Ceramic-based PCR and DNA Detection Devices”, IEEE TRANSACTIONS ON COMPONENTS AND PACKAGING TECHNOLOGIES, 26 (2), 309-316.

  • [44]     C.F. Chou*, R. Changrani, P. Roberts, D. Sadler, S. Lin, A. Mulholland, N. Swami, R. Terbrueggen, F. Zenhausern, 2002, “A Miniaturized Cyclic PCR Device-Modeling and Experiments”, MICROELECTRONIC ENGINEERING, 61/62, 921-925. (SCI) (IF: 1.277; SCI ranking: 62.8%,61.1%,49.8%,77.1%)

  • [45]     C.F. Chou, J.O. Tegenfeldt, O. Bakajin, S.S. Chan, E.C. Cox, N. Darnton, T.A.J. Duke, R.H. Austin , 2002, “Electrodeless Dielectrophoresis of Single and Double Stranded DNA”, BIOPHYSICAL JOURNAL, 83, 2170. (SCI) (IF: 3.632; SCI ranking: 25%)

  • [46]     J. O. Tegenfeldt, O. Bakajin, C. F. Chou, S. S. Chan, R. H. Austin, W. Fann, L. Liou, E. Chan, T. Duke, and E. C. Cox, 2001, “Near-Field Scanner for Moving Molecules”, PHYSICAL REVIEW LETTERS, 86, 1378-1381. (SCI) (IF: 7.645; SCI ranking: 7.6%)

  • [47]     O. Bakajin, T. A. J. Duke, J. Tegenfeldt, C. F. Chou, S. S. Chan, R. H. Austin and E. C. Cox , 2001, “Separation of 100 kilobase DNA molecules in 10 seconds”, ANALYTICAL CHEMISTRY, 73, 6053-6056. (SCI) (IF: 5.886; SCI ranking: 5.3%)

  • [48]     C.F. Chou, R.H. Austin, O. Bakajin, J.O. Tegenfeldt, J.A. Castelino, S.S. Chan, E.C. Cox, H.G. Craighead, N. Darton, T.A.J. Duke, J. Han, S.W. Turner , 2000, “Sorting Biomolecules with Microdevices”, Electrophoresis, 21, 81-90. (SCI) (IF: 2.482; SCI ranking: 34.7%,49.4%)

  • [49]     C.Y. Chao, T.C. Pan, C.F. Chou, and J.T. Ho , 2000, “Structural Characterization of Surface Hexatic Behavior in Free-Standing 4O.8 Liquid-Crystal Films”, PHYSICAL REVIEW E, 62 (2): R1485-R1488. (SCI) (IF: 2.252; SCI ranking: 11.3%,33.3%)

  • [50]     C.F. Chou, O. Bakajin, S.W. Turner, T. Duke, S.S. Chan, R.H. Austin, E.C. Cox, and H.G. Craighead , 1999, “Sorting by Diffusion: an Asymmetric Obstacle Course for Continuous Molecular Separation”, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 96, 13762-13765. (SCI) (IF: 9.423; SCI ranking: 6.3%)

  • [51]     O.B. Bakajin, T.A.J. Duke, C.F. Chou, S.S. Chan, R.H. Austin, and E.C. Cox , 1998, “Electrohydrodynamic Stretching of DNA in Confined Environments”, PHYSICAL REVIEW LETTERS, 80, 2737-2740. (SCI) (IF: 7.645; SCI ranking: 7.6%)

  • [52]     C.F. Chou*, A.J. Jin, S.W. Hui, C.C. Huang, and J.T. Ho , 1998, “Multiple-Step Melting in Two-Dimensional Hexatic Liquid-Crystal Films”, SCIENCE, 280, 1424-1426. (SCI) (IF: 34.661; SCI ranking: 3.2%)

  • [53]     M. Veum, C.C. Huang, C.F. Chou, and V. Surendranath , 1997, “Stability and Phase Transitions of Single-Molecular-Layer Free-Standing Liquid-Crystal Films”, PHYSICAL REVIEW E, 56, 2298-2301. (SCI) (IF: 2.252; SCI ranking: 11.3%,33.3%)

  • [54]     C.Y. Chao, S.W. Hui, J.E. Maclennan, C.F. Chou, and J.T. Ho , 1997, “Surface-Freezing Transitions and Novel Tilted Hexatic Phases in Smectic Liquid-Crystal Films”, PHYSICAL REVIEW LETTERS, 78, 2581-2584. (SCI) (IF: 7.645; SCI ranking: 7.6%)

  • [55]     C.Y. Chao, C.F. Chou, J.T. Ho, S.W. Hui, A.J. Jin, and C.C. Huang , 1996, “Nature of Layer-by-Layer Freezing in Free-Standing 4O.8 Films”, PHYSICAL REVIEW LETTERS, 77, 2750-2753. (SCI) (IF: 7.645; SCI ranking: 7.6%)

  • [56]     A.J. Jin, M. Veum, T. Stoebe, C.F. Chou, J.T. Ho, S.W. Hui, V. Surendranath, and C.C. Huang , 1996, “Nature of the Smectic-A–Hexatic-B–Crystal-B Transitions of One Liquid-Crystal Compound”, PHYSICAL REVIEW E, 53, 3639-3646. (SCI) (IF: 2.252; SCI ranking: 11.3%,33.3%)

  • [57]     C.F. Chou, J.T. Ho, S.W. Hui, and V. Surendranath , 1996, “Scaling of 6n-Fold Bond-Orientational Order Parameters in a Hexatic Liquid-Crystal Thin Film”, PHYSICAL REVIEW LETTERS, 76, 4556-4559. (SCI) (IF: 7.645; SCI ranking: 7.6%)

  • [58]     A.J. Jin, M. Veum, T. Stoebe, C.F. Chou, J.T. Ho, S.W. Hui, V. Surendranath, and C.C. Huang , 1995, “Calorimetric and Structural Characterization of Thin Liquid-Crystal Films Exhibiting the Smectic-A–Hexatic-B–Crystal-B Transitions”, PHYSICAL REVIEW LETTERS, 74, 4863-4866. (SCI) (IF: 7.645; SCI ranking: 7.6%)

學術會議(研討會)論文

  • [1]     B. J. Sanghavi, , W. Varhue, A. Rohani, J. L. Chavez, C. F. Chou, N. S. Swami* , 2014, “Conformation-Selective Enrichment of Aptamer-Bound Neuropeptides By Dielectrophoresis”, 2393-2395 pages, paper presented at 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (Micro Total Analysis Systems 2014), San Antonio, Texas, USA: The Chemical and Biological Microsystems Society (CBMS), 2014-10-26 ~ 2014-10-30.

獎項及殊榮

(1) 國內學術研究獎項 2014-04 2013 Outstanding Research Award of Ministry of Science and Technology

發現與突破

(1) 西元年:2012
研究人員(中):周家復、葉佳唯、Alessandro Taloni、陳彥龍
研究人員(英):CHOU, CHIA-FU, Jia-Wei Yeh, Alessandro Taloni, Yeng-Long Chen
研究成果名稱(中):微奈米流道介面熵力驅動之單分子DNA拔河
研究成果名稱(英):Entropy-driven single molecule tug-of-war of DNA at micro-nanofluidic interfaces
簡要記述(中):熵驅動的高分子動力學在生物系統中是極其重要的,但在奈米尺度上的熵力及其對奈米侷限度的相依性仍然是不清楚的。在這裡,我們建立了一個由熵力驅動的單分子DNA拔河系統,該系統由一個奈米狹縫橋接兩個微奈米流道的界面組成。由此系統,我們在不需施加外力的情況下,便可研究奈米尺度下,生物分子的熵力及其對奈米侷限度的相依性及其規度律。我們的結果提供了直接的實驗證據表明,熵力祇和分子在奈米尺度的空間侷限(即狹縫高度)相關,而和狹縫長度和在其內的DNA長度無關。我們的研究結果對高分子聚合物在奈米尺度的傳輸現象,單分子分析的系統設計上,及生物科技上均有潛在的應用性。
簡要記述(英):Entropy-driven polymer dynamics at the nanoscale is fundamentally important in biological systems but the dependence of the entropic force on the nanoconfinement remains elusive. Here we established an entropy-driven single molecule tug-of-war (TOW) at two micro-nanofluidic interfaces bridged by a nanoslit, performed the force analysis from a modified worm-like chain in the TOW scenario and the entropic recoiling process, and determined the associated scalings on the nanoconfinement. Our results provide a direct experimental evidence that the entropic forces in these two regimes, though unequal, are essentially constant at defined slit heights, irrespective of the slit lengths and the DNA segments within. Our findings have the implications to polymer transport at the nanoscale, device design for single molecule analysis, and biotechnological applications.
主要相關著作:
Jia-Wei Yeh, Alessandro Taloni, Yeng-Long Chen, Chia-Fu Chou*, 2012, “Entropy-driven single molecule tug-of-war of DNA at micro-nanofluidic interfaces (Research Highlighted by Nature)”, NANO LETTERS, 12 (3), 1597–1602. (SCI) (IF: 13.779; SCI ranking: 4.8%,4.3%,3%,2.8%,9%,3.6%)
(2) 西元年:2012
研究人員(中):周家復、廖國棠
研究人員(英):CHOU, CHIA-FU, Kuo-Tang Liao
研究成果名稱(中):奈米分子阱和分子壩應用於高導電度緩衝液內超快速之蛋白質富集(濃縮)
研究成果名稱(英):Nanoscale molecular traps and dams for ultrafast protein enrichment in high-conductivity buffers
簡要記述(中):我們提出一個新的分子富集的方法,稱為分子壩,該方法可在生理緩衝液內,以奈米尺度的無電極式介電泳,快速達到蛋白質富集與濃縮。我們以絕緣材料製作了奈米流道,並在其中嵌製30奈米大小的間隙,作為聚焦電場的透鏡,在結合微奈米流道界面時,可將外加電場放大約10萬倍。藉由這個奈米間隙聚焦的強電場和其場梯度,並利用分子壩的效應,我們達到快速濃縮蛋白質的效果,在20秒內便可濃縮至少10萬倍,比以往文獻報告的方法快了幾個數量級。我們的研究開闢了奈米級分子水壩的可能應用,包括以微型化感測平台快速且靈敏的分析蛋白質和發現生物標誌物,或應用於沉澱研究和蛋白質結晶學,並可能進一步擴展到小分子的富集或篩選。
簡要記述(英):We report a new approach, termed molecular dam, to enhance mass transport for protein enrichment in nanofluidic channels by nanoscale electrodeless dielectrophoresis under physiological buffer conditions. Dielectric nanoconstrictions, down to 30nm in size, embedded in nanofluidic device, serve as field focusing lens capable of magnifying the applied field to 100000-fold when combined with a micro-to-nanofluidic step interface. Empowered by this strong field and the associated field gradient occurred at the nanoconstrictions, we demonstrate proteins are enriched by molecular damming effect, faster than the trapping effect, to greater than 100000-fold in 20 seconds, which is orders of magnitude faster than most reported methods. Our study opens up further possibilities of using nanoscale molecular dams in miniaturized sensing plat-forms for rapid and sensitive protein analysis and biomarker discovery, with potential applications in precipitation studies and protein crystallization, and possible extensions to small molecules enrichment or screening.
主要相關著作:
Kuo-Tang Liao, Chia-Fu Chou*, 2012, “Nanoscale molecular traps and dams for ultrafast protein enrichment in high-conductivity buffers (Featured in JACS Spotlights)”, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 134 (21), 8742−8745. (SCI) (IF: 13.038; SCI ranking: 6.1%)
個人資料維護 | 著作目錄維護同步更新 | 最後更新日期 : 2017-09-11
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