Introduction to Nano-biophotonics

Instructor: Chia-Fu Chou 周家復
Time: Wed. 9:10-12:00
Classroom: be announced
Office hours: Mon. 10-12 @ P612, IoP

Introdution:　　　　　　　　　　　　　　　　　　　　　　　　　　

In this course, we will review the basics and recent developments of nanobiophotonics, an emerging field which is cross-displinary among nanoscience and nanotechnology, biology, and photonics. The course is comprised of lectures from several special topics given by experts in these areas, including micro/nanofluidics, single molecule biophysics, DNA microarray, polymer brush, optical tweezers, near-field optics, plasmonics (SPR, SERS), advanced bioimaging techniques (fluorescence, confocal, CARS, TIRFM, FRET, higher harmonics, multiphoton, X-ray nanoimaging, super-high resolution imaging, etc.), image analysis and lab tours. Grading will be based on term project presentation and report.

Grade evaluation:

Term project presentation–50%

Written report (no more than 10 pages, due 1/12/09)–50%

Attendance:

Students will need to sign the attendance sheet for each class. Any student who has 2 no-shows will be failed from the course. The instructor should be informed in advance if medical absence or other emergency matters occurred.

Speakers and topics to be covered:

Chang, Wei-Hau 章為皓(1) – Single Molecule FRET

Cheng, Ji-Yen 鄭郅言 (1) – DNA microarrays-fabrication, detection, and analysis

Chou, Chia-Fu 周家復 (7) – Introduction, micro/nanofluidics, SM biophys, bioimaging, Fluorescence microscopy, TIRFM, Term project assignment & evaluation

Gukassyan, Vladimir (1) (YMU) – Time-resolved fluorescence microscopy (FLIM, FCS, FRET, TCSPC)

Hwu, Yeu-Kuang 胡宇光(1) – X-ray nanoimaging

Juan, Wen-Tau 阮文滔(1) Polymer brush, optical tweezers

Lee, Chau-Hwang 李超煌 (1) – Confocal, NIWOP-cell membrane imaging, image processing

Tang, Jau 湯朝暉(1) – Studies of single molecules using confocal microscopy; photophysical properties of quantum dots; studies of electron and energy transfer reactions; single-enzyme catalytic reactions; ultrafast electron diffraction

Wei, Pei-Kuen 魏培坤 (1) – Near-field optics/SNOM, SERS, Nanoscale optical control and biosensing

Yang, Wei-Yuan 楊維元(1)– CARS Microscopy

 

Class Schedule for Fall Semester, 2008

Week	Wednesday	9:10-12:00	Week	Wednesday	9:10-12:00
1	97/09/24	Lee, Chau-Hwang	9	97/11/19	Tang, Jau
2	97/10/01	Chou, Chia-Fu	10	97/11/26	Chang, Wei-Hau
3	97/10/08	Chou, Chia-Fu	11	97/12/03	Yang, Wei-Yuan
4	97/10/15	Wei, Pei-Kuen	12	97/12/10	Cheng, Ji-Yen
5	97/10/22	Gukassyan, Vladimir	13	97/12/17	Student presentation Chou, Chia-Fu
6	97/10/29	Hwu, Yeu-Kuang	14	97/12/24	Student presentation Chou, Chia-Fu
7	97/11/05	Term project ssignment / Lab tour Chou, Chia-Fu	15	97/12/31	Student presentation Chou, Chia-Fu
8	97/11/12	Juan, Wen-Tau	16	98/01/07	Student presentation Chou, Chia-Fu

 Reference:

1. Optical microscopy in general- http://micro.magnet.fsu.edu/
2. Stefan A. Maier: Plasmonics: Fundamentals and Applications (Springer, 2007)
3. Lukas Novotny and Bert Hecht: Principles of Nano-Optics (Cambridge University Press, 2006)
4. Joseph R. Lakowicz: Principles of Fluorescence Spectroscopy (3rd Ed., Springer, 2006)
5. Alastair Smith, Christopher Gell, and David Brockwell: Handbook of Single Molecule Fluorescence Spectroscopy (Oxford University Press, 2006)
6. Katrin Kneipp, Martin Moskovits, and Harald Kneipp: Surface-Enhanced Raman Scattering: Physics and Applications (Springer, 2006)
7. Wolfgang Becker: Advanced Time-Correlated Single Photon Counting Techniques (Springer Series in Chemical Physics, 2005)
8. Christof M. Niemeyer and Chad A. Mirkin (Editors): Nanobiotechnology: Concepts, Applications and Perspectives (Hardcover, Wiley-VCH, 2004)
9. Roland Glaser: Biophysics (Springer, 2004)
10. Paras N. Prasad: Introduction to Biophotonics (Wiley-Interscience, 2003)
11. West JL, Halas NJ: Engineered nanomaterials for biophotonics applications: Improving sensing, imaging, and therapeutics. Annual Review of Biomedical Engineering 5: 285-292 2003.
12. H. Berg, The Random Walk in Biology, Princeton University Press, 1993.

 Term project topics: (term project report due 1/12/09)

 Topics:

• Precise nanometer localization analysis for individual fluorescent probes.
  Biophys. J. 82: 2775-2783, 2002. (Paul Cardenas)
• Single-Molecule Analysis 2: Single-Molecule Fluorescence Resonance Energy
  Transfer. TJ Ha. METHODS 25, 78–86, 2001. (Fei Pei)
• FRET imaging. Nature Biotech. 21: 1387-1395, 2003. (Li-Wei Huang, Chih-
  Ning Chen)
• FRET or no FRET: A quantitative comparison. Biophys. J. 84: 3992–4010,
  2003. (Mohamed Abbass, Mohamad Yaseen)
• Single-quantum-dot-based DNA nanosensor. Nature Materials 4, 826-831,
  2005. (Chia-Jong Liu, Meng-Lin Tsai)
• Resolution scaling in STED microscopy. Harke et al., OPTICS EXPRESS 16,
  4154-4162, 2008. (Kumar Sharma)
• Biolabeling with nanoparticles (gold NPs, QDs, and nanodiamonds) (Chen-
  Hon Nee, Huai-Yi Chen)
• CNT biosenor: Cellular uptake of functionalized carbon nanotubes is
  independent of functional group and cell type. Nature Nanotechnology, 2,
  108–113, 2007. (Bhagava Billa)
• Photo-nanobiotechnology: Quantum dots and nanoparticles for
  photodynamic and radiation therapies of cancer. Advanced Drug Delivery
  Reviews 2008 (in press); Trends in Biotechnology Vol.26 No.11 (in press,
  doi:10.1016/j.tibtech.2008.07.007). (Tony Rajan)

97/12/17 (W)	1. Abbass/Yaseen 2. Pei. 3. Toney
97/12/23 (Tu.)	1. Paul 2. Li-Wei/Chih-Ning
97/12/24 (W)	1. Chia-Jong/Meng-Lin 2. Chen-Hon/Huai-Yi
97/12/31 (W)	1. Billa 2. Kumar

Classnote:

2008 / 9 / 24	Modern Optical Microscopy
2008 / 10 / 15	Surface Plasmon_1 Surface Plasmon_2
2008 / 10 / 22	Time-Resolved Mocroscopy
2008 / 11 / 12	Optical Tweezers
2008 / 11 / 19	Studies of light-induced stochastic and coherent processes in nano-structured materials using single-molecule techniques and femtosecond time-resolved electron diffraction